Molar Mass of EDTA

The molar mass of EDTA (C10H16N2O8) is 292.244 g/mol, derived element by element, with composition by mass.

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The molar mass of EDTA, C10H16N2O8, is 292.244 g/mol. That single number is what connects a mass you can weigh to an amount of substance you can react: one mole is 292.244 g, and a gram of it is 2.061e+21 formula units. The calculator turns any weighed mass into that count, and reports the amount in moles, millimoles and the mass of a single formula unit alongside it.

The figure comes from the formula. EDTA is 10 × 12.011 (C) + 16 × 1.008 (H) + 2 × 14.007 (N) + 8 × 15.999 (O), which is 292.244 g/mol, taking standard atomic weights, and every page here computes that sum rather than quoting it. Oxygen makes up the largest share of the mass: 8 of the 36 atoms in a formula unit are oxygen, and they account for 127.992 g of the 292.244 g, or 43.8% by mass. The derivation table below breaks the whole molecule down element by element.

The numbers a bench actually uses sit at the millimole scale. One millimole of EDTA is 292.244 mg and one micromole is 292.244 µg, so a balance reading to a milligram places you within 3.422 µmol. A single formula unit weighs 4.8528e-22 g, which is why the count in the gauge runs into the sextillions for any mass you can see.

The chelator that binds metal ions one to one, used in blood tubes, shampoos and titrations. The free acid barely dissolves; the disodium salt does. At 292.244 g/mol it is heavier than 18 of the 21 organic compounds covered here, and that ranking matters more than it looks: caffeine has a molar mass of 194.194 g/mol, so a gram of it contains 50.5% more formula units than a gram of EDTA. Weigh by mass and you are not weighing equal amounts of substance.

Purity is the usual gap between the calculation and the balance. A reagent sold at 98% means 5.8449 g of a nominal 292.244 g is something else, so for exact work you divide the weighed mass by the assay figure on the certificate. For most purposes the difference is smaller than the error in reading the meniscus, but it is systematic rather than random, so it does not average out.

The formula

N = m ÷ 292.244 × 6.02214076×1023
m
The mass of EDTA in grams
292.244
The molar mass of EDTA in g/mol, derived from its formula
N
The number of formula units that mass contains

How it works, step by step

  1. Enter a mass of EDTA in grams.
  2. It is divided by the molar mass, 292.244 g/mol, giving the amount in moles.
  3. That amount is multiplied by the Avogadro constant, 6.02214076 × 10²³ per mole, to give the number of formula units.
  4. The panel also reports the amount in moles and millimoles and the mass of one formula unit.

Worked examples

0.5 g of EDTA as a molecule count

0.5 g divided by 292.244 g/mol is 0.0017109 mol, and multiplying by the Avogadro constant gives 1.03e+21 formula units of EDTA. The same mass is 1.7109 mmol, which is the figure a reaction is actually planned in.

5 g of EDTA as a molecule count

5 g divided by 292.244 g/mol is 0.017109 mol, and multiplying by the Avogadro constant gives 1.03e+22 formula units of EDTA. The same mass is 17.109 mmol, which is the figure a reaction is actually planned in.

50 g of EDTA as a molecule count

50 g divided by 292.244 g/mol is 0.17109 mol, and multiplying by the Avogadro constant gives 1.03e+23 formula units of EDTA. The same mass is 171.09 mmol, which is the figure a reaction is actually planned in.

How to read your score

0–0NothingNo mass, no molecules.
0–—Milligram scaleUnder 2.061e+18 formula units — the amount in a milligram or less of EDTA. Still an enormous count, which is the point of the mole.
—–—Bench scaleThe range from a milligram to ten grams, where nearly all weighing of EDTA is done.
—–—Bulk scaleMore than ten grams. Beyond a few hundred grams the count is better handled in moles than in formula units.

Frequently asked questions

What is the molar mass of EDTA (C10H16N2O8)?

It is 292.244 g/mol. That is the sum of the standard atomic weights of every atom in the formula: 10 × 12.011 (C) + 16 × 1.008 (H) + 2 × 14.007 (N) + 8 × 15.999 (O). One mole of EDTA therefore weighs 292.244 g, and a gram of it is 3.4218 mmol.

What percentage of EDTA is oxygen?

43.8% by mass. Each formula unit contains 8 oxygen atoms contributing 127.992 g of the 292.244 g total, so 100 g of EDTA contains 43.8 g of oxygen and a kilogram contains 437.96 g of it.

How many molecules are in one gram of EDTA?

About 2.061e+21. One gram is 0.0034218 mol, and each mole contains 6.02214076 × 10²³ formula units by definition of the mole, so the count is that constant divided by 292.244.

How much does a single molecule of EDTA weigh?

4.8528e-22 g, which is 292.244 daltons. It is the molar mass divided by the Avogadro constant, and the number in daltons is numerically the same as the molar mass in g/mol — that equivalence is what makes the mole convenient.

Is molar mass the same as molecular weight?

They are the same number in ordinary use but not the same quantity. Molecular weight, properly relative molecular mass, is a ratio and has no unit: for EDTA it is 292.244. Molar mass carries grams per mole: 292.244 g/mol. Because the mole is defined so those agree, you can read one off the other.

Why is the molar mass not a whole number?

Because standard atomic weights are averages over the isotopes found in nature. Oxygen is quoted as 15.999 rather than a whole number for that reason, and the same applies to the other elements here, which is why EDTA comes out at 292.244 g/mol rather than 292.

Molar mass, composition and mole equivalents of EDTA

How the molar mass of EDTA is arrived at
ElementAtomsAtomic weightContribution (g/mol)By mass
Oxygen (O)815.999127.99243.8%
Carbon (C)1012.011120.1141.1%
Nitrogen (N)214.00728.0149.586%
Hydrogen (H)161.00816.1285.519%
Total — one mole of EDTA292.244100%

Standard atomic weights, IUPAC 2021. The contribution column is atoms × atomic weight, and the total is the molar mass this page uses: 292.244 g/mol.

Mole quantities of EDTA and what they weigh
AmountMass (g)Mass (mg)Formula units
1 µmol0.0002922440.2922446.022e+17
10 µmol0.002922442.922446.022e+18
100 µmol0.029224429.22446.022e+19
1 mmol0.292244292.2446.022e+20
10 mmol2.922442922.446.022e+21
50 mmol14.612214,612.23.011e+22
100 mmol29.224429,224.46.022e+22
250 mmol73.06173,0611.506e+23
500 mmol146.122146,1223.011e+23
750 mmol219.183219,1834.517e+23
1 mol292.244292,2446.022e+23
2 mol584.488584,4881.204e+24
5 mol1461.221461220.0000003.011e+24
10 mol2922.442922440.0000006.022e+24

Mass is the amount multiplied by 292.244 g/mol. The last column is that amount multiplied by the Avogadro constant, 6.02214076 × 10²³ per mole.

EDTA among other organic compounds, by molar mass
CompoundFormulaMolar mass (g/mol)Millimoles in 1 g
Uric acidC5H4N4O3168.1125.9484
GlucoseC6H12O6180.1565.5507
AspirinC9H8O4180.1595.5507
CaffeineC8H10N4O2194.1945.1495
EDTA (this page)C10H16N2O8292.2443.4218
SucroseC12H22O11342.2972.9214
CholesterolC27H46O386.6642.5862

Ordered by molar mass. The last column is 1000/M, which is the number a weighed gram actually gives you.

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